David J Carroll

Microinjection: Methods and Applications - Methods in Molecular Biology David J Carroll 2009 edition

Marc Notes: Includes bibliographical references and index. Table of Contents: Preface -- Contributors -- 1. Expression of Exogenous mRNA in Xenopus laevis Embryos for the Study of Cell Cycle Regulation / Jill C. Sible, Brian N. Wroble -- 2. Use of Luciferase Chimaera to Monitor PLC[zeta] Expression in Mouse Eggs / Karl Swann, Karen Campbell, Yuansong Yu, Christopher Saunders, F. Anthony Lai -- 3. Analysis of 14-3-3 Family Member Function in Xenopus Embryos by Microinjection of Antisense Morpholino Oligos / Jeffrey M. C. Lau, Anthony J. Muslin -- 4. A Microinjectable Biological System, the Xenopus Oocyte, as an Approach to Understanding Signal Transduction Protein Function / Katia Cailliau, Edith Browaeys-Poly -- 5. Combining Microinjection and Immunoblotting to Analyze MAP Kinase Phosphorylation in Single Starfish Oocytes and Eggs / David J. Carroll, Wei Hua -- 6. Analysis of Signaling Pathways in Zebrafish Development by Microinjection / William H. Kinsey -- 7. Protein Inhibition by Microinjection and RNA-Mediated Interference in Tissue Culture Cells: Complementary Approaches to Study Protein Function / Jane R. Stout, Rania S. Rizk, Claire E. Walczak -- 8. DNA Delivery by Microinjection for the Generation of Recombinant Mammalian Cell Lines / Sebastien Chenuet, Madiha Derouazi, David Hacker, Florian Wurm -- 9. Bacteriophage [phi]C31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels / Bryan G. Allen, Daniel L. Weeks -- 10. Germline Transformation of Caenorhabditis elegans by Injection / Pavan Kadandale, Indrani Chatterjee, Andrew Singson -- 11. Quantitative Microinjection of Mouse Oocytes and Eggs / Douglas Kline -- 12. Microinjection of Follicle-Enclosed Mouse Oocytes / Laurinda A. Jaffe, Rachael P. Norris, Marina Freudzon, William J. Ratzan, Lisa M. Mehlmann -- 13. Functional Studies of Regulatory Genes in the Sea Urchin Embryo / Vincenzo Cavalieri, Maria Di Bernardo, Giovanni Spinelli -- 14. Exploring the Cytoskeleton During Intracytoplasmic Sperm Injection in Humans / Vanesa Y. Rawe, Hector Chemes -- 15. Somatic Cell Nuclear Transfer in the Mouse / Satoshi Kishigami, Teruhiko Wakayama -- Index. Review Quotes: From the reviews: "Chapters cover all of the hot topics in which the technique can be used (namely, all the research field you are involved!) and so many different model systems associated with other master technique (e.g., western blot analysis). the beginners can immediately get good self-trainings tricks that will ensure them to reach good results. I would like all the readers to get a copy of the book: I am sure they will broad the paraphernalia usefull to carry on succesfull researches." (CarloAlberto Redi, European Journal of Histochemistry, October, 2009)"Jacket Description/Back: As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research."Review Quotes: From the reviews: "Chapters cover all of the hot topics in which the technique can be used (namely, all the research field you are involved!) and so many different model systems associated with other master technique (e.g., western blot analysis). the beginners can immediately get good self-trainings tricks that will ensure them to reach good results. I would like all the readers to get a copy of the book: I am sure they will broad the paraphernalia usefull to carry on succesfull researches." (CarloAlberto Redi, European Journal of Histochemistry, October, 2009) Publisher Marketing: In 'Microinjection', expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. Review Citations:

Scitech Book News 06/01/2009 pg. 54 (EAN 9781588298843, Hardcover)