Aleksandr Nikolaevich Orekhov

Aterogennost' Lipoproteidov: Mekhanizmy, Rol' Assotsiatsii Aleksandr Nikolaevich Orekhov Russian edition

Byli izucheny prichiny aterogennosti tsirkuliruyushchikh v krovi bol'nykh modifitsirovannykh lipoproteidov nizkoy plotnosti (tsmLNP). Pokazano, chto tsmLNP sklonny k obrazovaniyu assotsiatov. Ustanovleno, chto mezhdu stepen'yu assotsiatsii tsmLNP i ikh aterogennym potentsialom sushchestvuet pryamaya zavisimost'. Izucheny protsessy zakhvata i degradatsii agregatov tsmLNP kletkami intimy. Ustanovleno, chto assotsiaty tsmLNP zakhvatyvayutsya kletkami putem nespetsificheskogo fagotsitoza. Ispol'zovanie paneli monoklonal'nykh antitel k apo-V-100 pozvolilo obnaruzhit' lokal'nye izmeneniya v belkovoy chasti tsmLNP po sravneniyu s nativnymi LNP, kotorye mogut yavit'sya prichinoy snizheniya ikh ustoychivosti k assotsiatsii. Issledovany osobennosti raspolozheniya antigennykh determinant apoV-100 v tsmLNP i LNP, modifitsirovannykh in vitro proteoliticheskimi, lipoliticheskimi, okislitel'nymi, desialiruyushchimi fermentami, a takzhe al'degidami endogennogo proiskhozhdeniya. Pokazano, chto modifikatsiya LNP perechislennymi vyshe agentami vyzyvaet assotsiatsiyu LNP. Vyyavleno ingibiruyushchee deystvie plyuronikov na assotsiatsiyu LNP; izucheno vliyanie struktury i svoystv plyuronikov na sposobnost' podavlyat' assotsiatsiyu LNP.