Monzur Hossain

Microtuberization in Potato: Physical & Chemical Factors on Induction & Characterization of Microtubers and Nuclear Seed Production in Potato Monzur Hossain

Different sugar types were evaluated on microtuberization efficiencies of potato and sucrose was considered to be the best sugar type and 8% sucrose in media was optimized. The media with 5 uM Jasmonic acid resulted the highest number and maximum yield of microtubers than the other growth regulators used and high nitrate content media increased microtuber size. Application of 2 mg/L AgNO3 and propionic acid added media favored the in vitro microtubers formation. Microtuberization in liquid media followed by incubation at 8 h photoperiod promoted the number and fresh weight of microtubers than the other physical supports (agar based, liquid plus cotton and liquid plus sponge) and incubation (0, 8 and 16 h). The larger microtubers (0.5-0.6 cm diameter) showed significantly higher survival capacity than smaller ones (0.3-0.4 cm dia). The dormancy termination and sprouting efficiencies of microtubers were noted best in media with GA3 (100 ppm) treatment, large size of microtubers and dark incubation. The media with 5 uM Jasmonic acid showed maximum accumulation of total sugar, reducing sugar and starch. The vitamin C content of microtubers decreased with the extended storage periods.